Adipocyte single cell sequencing
WebNov 8, 2024 · Adipose tissue has been classified based on its morphology and function as white, brown, or beige/brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology … WebApr 27, 2024 · Single-cell sequencing of human white adipose tissue identifies new cell states in health and obesity Main. WAT is the primary metabolic organ utilized for energy storage in mammals. During dietary nutrient excess,... Results. To understand the … Metrics - Single-cell sequencing of human white adipose tissue ... - Nature Extended Data Fig. 4 Flow Cytometry Analysis of scRNAseq-identified Human … Extended Data Fig. 3 Single Cell Analysis Identifies Unique Human WAT-resident …
Adipocyte single cell sequencing
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Web1 day ago · A high-throughput single-cell single-mitochondrial genome sequencing technology known as iMiGseq has provided new insights into mutations of mitochondrial DNA (mtDNA) and offers a platform for ... WebSpatial and single-cell transcriptomics identify main contributors of ECM in iWAT. To identify which adipose tissue cell types are mainly responsible for ECM remodeling, we reanalyzed previously published single-cell RNA sequencing datasets of both adipose stromal-vascular and mature adipocyte fractions 6, 28 (Figures S2 A and S2C).
Web1 day ago · A high-throughput single-cell single-mitochondrial genome sequencing technology known as iMiGseq has provided new insights into mutations of mitochondrial … WebApr 12, 2024 · The stem cell niches driving neuroregeneration and their molecular signatures remain elusive. To investigate this, we performed scRNA-seq of the adult telencephalon, combined with full-length transcriptomics using ISO-seq. Our results unveil about 25 cell types including neurons and progenitors of glial-and non-glial nature.
WebDec 21, 2024 · Single nucleus sequencing (snRNAseq) is a modified version of scRNAseq, wherein a single nucleus is sequenced for actively transcribed RNA instead of a single cell. This technique is especially effective on tissues that cannot be dissociated into intact single cells, such as adipocytes. WebFeb 10, 2024 · Halfway through maturation, these cells have a brown adipocyte signature regardless of adipose depot origin. The structural cell type arises from the ... We used single-cell RNA sequencing (scRNA-seq) and performed temporal modeling of the transcriptional data to track transcriptional branching of cellular subtypes during …
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WebJun 6, 2024 · Unsorted adipocyte nuclei contain debris and doublets that create noise and high background in downstream single-cell RNA sequencing. The representative FACS … listwidget currentrowWebAdipocytes arise from an existing pool precursor niche, which is a cluster of committed preadipose fibroblasts maintained by self-replication. With proper adipogenic stimuli, … listwidget countWebApr 12, 2024 · Single-cell sequencing would allow you to examine and identify the components of each fruit (strawberries, oranges, kiwis, grapes). This gives a very clear picture of the genomes from each cell type and allows you to identify a potential gene, or target, that can only be found in the strawberries so that whatever therapy or drug you … impa twilight princessWebMay 7, 2024 · Single-cell sequencing is valuable for the study of the heterogeneity of highly complex cell populations and in the analysis of their cell subpopulations as well as changes in their molecular mechanisms [30,31,32,33]. The main procedure involves establishing a library of multiple single-cells independent from each other with their … list wifi networks windows 10WebNov 7, 2024 · Using unbiased single cell transcriptomics we aimed to characterize ASC populations in human subcutaneous white adipose tissue (scWAT). The transcriptomes of 574 single cells from the WAT total stroma vascular fraction (SVF) of four healthy women were analyzed by clustering and t-distributed stochastic neighbor embedding visualization. listwidget currentindeximpa\\u0027s house botwWebNov 18, 2024 · Osteoblasts and osteocytes are derived from bone marrow mesenchymal stem cells (MSCs), which also give rise to marrow adipocytes. Recently, we computationally delineated the hierarchy of mesenchymal lineage cells from MSCs to mature cells using large-scale, single-cell RNA-sequencing (scRNA-seq). listwidget remove