Dsred-express2

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August undefined, 2024

http://pharmbiology.com/page9?product_id=642 WebPlasmid pSIR-DsRed-Express2 from Dr. Hodaka Fujii's lab is published in PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014. This plasmid is available through Addgene. Image: …

DsRed-Express2 Monoclonal Antibody (OTI1C4) (TA180014)

WebDsRed-Express2 Antibody (TA180014) in WB. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DsRed-Express2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DsRed-Express2 (TA180014, 1:2000). WebApr 3, 2009 · Background: Whole-cell labeling is a common application of fluorescent proteins (FPs), but many red and orange FPs exhibit cytotoxicity that limits their use as whole-cell labels. Recently, a tetrameric red FP called DsRed-Express2 was engineered for enhanced solubility and was shown to be noncytotoxic in bacterial and mammalian cells. oftalmoplegie

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WebOct 17, 2014 · To generate mice expressing DsRed Express2 under the neuron specific enolase promoter (NSE-DsRedE2) a cDNA construct was made by replacing the cytomegalovirus (CMV) promoter in the pCMV DsRed ... WebpIRES2 DsRed-Express 2 is a eukaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV) between the MCS and red fluorescent protein DsRed-Express2 coding region.This permits both the gene of … WebOct 26, 2008 · DsRed-Express2 has additional advantages. It is excited efficiently by both blue and green lasers that are routinely used in flow cytometers. In bacteria, DsRed-Express2 is expressed from the ... oftalmoplejia internuclear neurowikia

A noncytotoxic DsRed variant for whole-cell labeling - Nature

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Web产品应用. FDA 是一种非荧光性疏水性荧光素衍生物，它可以穿透细胞膜进入细胞，通过细胞内酯酶催化水解二乙酸酯基团产生具有高强度的荧光产物荧光素。. 荧光分子在具有完整细胞膜的细胞内积累，因此这种绿色荧光可以作为一种细胞活力的标签。. 不具有 ... WebDsRed-Express2 is not as bright as wild-type DsRed, so we mutagenized it to create DsRed-Max, which was B30% brighter than DsRed-Express2 and retained high solubility (Fig. 1b and Supplementary Results ). DsRed-Max had lower green emission than DsRed-Express2 (Supplementary Fig. 6 online), but was slower to mature and more photolabile … oftalmoplejia completaWebTo overcome the slow maturation of DsRed, we used random and directed mutagenesis to create variants that mature 10–15 times faster than the wild-type protein.An asparagine-to-glutamine substitution at position 42 … my french is rusty in french

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Dsred-express2

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WebMar 9, 2011 · Here, we report that DsRed and its variant DsRed-Express2 inhibit the expression of B-cell lymphoma-extra large (Bcl-xL) in HeLa cells by translational regulation. Over-expression of Bcl-xL can reduce the cytotoxicity of DsRed. Meanwhile, Turbo RFP, a mutant RFP from Entacmaea quadricolor, does not affect Bcl-xL expression, suggesting … WebpEF1α-IRES-DsRed-Express2哺乳动物表达载体说明; FTTH光缆线路工程毕业设计; 聚苯硫醚的供需现状和产业进展; 心血管活动的调节----神经调节 (1) 2013《旅游目的地概述》培训考核大纲

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WebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA … WebFor example, the DsRed-Express variant (available from Clontech) can be detected within an hour after transfection of cells, compared to approximately six hours for DsRed2 and 11 to 15 hours for DsRed. More advanced versions of DsRed, named DsRed-Express2 and DsRed-Max, exhibit faster maturation rates and improved solubility.

WebJun 3, 2024 · Therefore,we selected DsRed-Express2 as an internal control (the second signal)in yeast cells to normalize the cell number and the cell state.DsRed-Express-2 is a noncytotoxic DsRed(Discosoma sp. red fluorescent protein) variant that retains favorable properties,such as brightness, fast maturation, and photostability, of this protein.In this ... WebPlasmid pSIR-DsRed-Express2 from Dr. Hodaka Fujii's lab is published in PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to ...

WebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using the curated feature database. Store, search, and share your sequences, files and maps. WebdsRED-express2 eGFP. Use. Cre/Lox and Lentiviral Tags. Expression. Mammalian . Mutation. Promoter. CMV. Availability. Academic Institutions and Nonprofits only Enlarge These results may also be relevant. pAC572. Plasmid #127973. Purpose. AMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI) ...

WebThe gene encoding DsRed-Express2 is fused to a constitutive promoter of GPD, providing an internal control for normalizing cell numbers in the assay. The dual fluorescent protein assay system is performed by sequentially measuring the yEGFP and DsRed-Express2 fluorescent intensity of the same sample, with the results expressed as the ratio of ...

WebDsRed-Express2 is a basic (constitutively fluorescent) orange fluorescent protein published in 2008, derived from Discosoma sp.. It is reported to be a rapidly-maturing protein. We would like to show you a description here but the site won’t allow us. Allison Berman - DsRed-Express2 :: Fluorescent Protein Database Wen Tao - DsRed-Express2 :: Fluorescent Protein Database Hal E Broxmeyer - DsRed-Express2 :: Fluorescent Protein Database References and fluorescent proteins attributed to Dibyendu Bhattacharyya at … Browse. If you just want to browse the database without searching something … A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell … References and fluorescent proteins attributed to Daniel E Strongin at … A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell … myfrenchkeyringWebAntibodies that detect DsRed-Express2 can be used in several scientific applications, including Western Blot. Our DsRed-Express2 monoclonal antibodies are developed in … oftalmoplejia internuclear derechaWebApr 3, 2009 · Whole-cell labeling is a common application of fluorescent proteins (FPs), but many red and orange FPs exhibit cytotoxicity that limits their use as whole-cell labels. … my french knotWebDsRed-Express & DsRed-Express2. DsRed-Express is a rapidly maturing variant of Discosoma sp. red fluorescent protein (DsRed) with mutations that enhance its solubility, … oftalmoplejia wernickeWebpIRES2 DsRed-Express 2 is a eukaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein contains the internal ribosome entry … oftalmoplejia intranuclearWebDec 5, 2005 · Directed evolution of DsRed-Express2 yielded the color variants E2-Orange, E2-Red/Green, and E2-Crimson. These variants can be used to label whole cells for single- and multi-color experiments ... my french lab and masteryWebPlasmid pDL278_P23-DsRed-Express2 from Dr. Robert Shields's lab contains the insert DsRed-Express2 and is published in Appl Environ Microbiol. 2024 May 17. pii: AEM.00620-19. doi: 10.1128/AEM.00620 … my frenchie tomboy