Phic31 attp site

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August undefined, 2024

WebOct 8, 2024 · An engineered phiC31 "Disintegrase" able to make an attP site in Drosophila out of an attR-attL pair is described. This was used to generate attP sites at genomic …

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WebThe phiC31 integrase catalyzes unidirectional recombination between two attachment sites, attP and attB, leading to the formation of attL and attR sites . A circular construct harboring an attB site can be efficiently and specifically integrated into … WebAug 20, 2024 · Finally, the candidate Phic31 pseudo attP site located in chromosome 6 was identified as an optimal integration site compared to the pseudo attP site of chromosome … pse xlr 900 bow

Using a phiC31 “Disintegrase” to make new attP sites in …

WebEnter the email address you signed up with and we'll email you a reset link. WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … WebMay 3, 2007 · ϕC31 attB and attP sites. (A) The double-stranded DNA sequences of the S. coelicolor attB site (green) and the attP site (blue) are shown.The crossover dinucleotides are shown in black. The colons connecting the two sequences indicate the positions of sequence identity between the aligned attB and attP sites. The grey shading indicates the … horse smiles in picture

phiC31-integrase-mediated, site-specific ... - Kyushu University

WebSequences of PhiC31 and BxB1 attachment sites For each recombinase, the attB and attP attachment sites share a common central dinucleotide sequence (in red) that is used for … WebOct 8, 2024 · One is a mutagenized phiC31 Integrase protein capable of mediating recombination between attR and attL sites to yield an attP site. The other is a P-element … pse xforce specsWebApr 8, 2024 · For example, we have developed the phiC31 integrase system for use in mammalian cells. This phage integrase pairs two short recognition sites, called attB and attP, and catalyzes recombination that results in chromosomal integration. This figure shows one approach we have developed with phiC31 integrase. horse smith game

"WebAug 20, 2024 · PhiC31 integrase-based vectors can integrate therapeutic genes selectively into attP or pseudo-attP sites in genomes, but considerable numbers of pseudo-attP sites … " - Phic31 attp site

Phic31 attp site

WebJan 14, 2024 · PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31 integrase which mediates sequence-directed, irreversible … WebSep 16, 2008 · In this method, which we term “site-specific integrase mediated repeated targeting” (SIRT), an attPattachment site for the phage phiC31 integrase is first targeted to the vicinity of the gene of interest by homologous recombination.

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WebPhiC31 integrase, a site-specific large serine recombinase, is a useful tool for genome engineering in a variety of eukaryotic species and cell types. PhiC31 integrase performs efficient recombination between its attB site and either its own placed attP site or a partially mismatched genomic pseudo attP site. WebJun 17, 2010 · Unlike Cre and Flp, phiC31 recognizes two heterotypic sites, attB and attP, for recombination. While the phiC31 system has been recently applied in mouse and human cell lines and in Drosophila, it has not been demonstrated whether it can also catalyze efficient DNA recombination in zebrafish.

WebExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism Depositor Norbert Perrimon WebApr 1, 2004 · Abstract. The φC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and mammalian cells, mediating unidirectional site-specific recombination between its attB and attP recognition sites. Here we show that this site-specific integration system also functions efficiently in Drosophila melanogaster in cultured cells and in …

WebJun 12, 2024 · The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific genomic integration in mammalian cells, recombining donor plasmids bearing the attB recognition site with introduced genomic attP sites or endogeneous pseudo- attP sites having partial identity to attP. WebPhiC31 integrase [ edit] Without much doubt, Ser integrases are the current tools of choice for integrating transgenes into a restricted number of well-understood genomic acceptor sites that mostly (but not always) mimic the phage att P site in that they attract an att B-containing donor vector.

WebJul 25, 2024 · The PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, …

WebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB … horse smiths cornerWebMay 5, 2013 · Background: PhiC31 integrase is a DNA site-specific recombinase integrates DNA into the chromosomes between the two sites of attB and attP. Several pseudo attPs have been identified in mammalian ... horse smzWebphiC31 integrase is a site-specific recombinase from a bacteriophage that has become a useful tool in mammalian cells. The enzyme normally performs precise, unidirectional … horse snacksWebDec 1, 2024 · Three novel PhiC31 pseudo-attP sites were identified on chromosomes 1, 3 and 6, and their genomic features were analyzed. The characterized platform cell lines are stable, and because of single-copy, site-specific R4 recognition attP site, the cell lines could be retargeted for recombinant protein production and drug discovery applications. horse snake compatibilityWebThe phiC31 integrase system efficiently integrates plasmid DNA carrying a transgene and an attB site into a limited number of endogenous pseudo attP sites in mammalian genomes, leading to robust ... pse xo crossbow scopeWebSequences of PhiC31 and BxB1 attachment sites For each recombinase, the attB and attP attachment sites share a common central dinucleotide sequence (in red) that is used for the strand exchange... pse xlr-900 infinity compound bowWebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … pse xforce gx string length