Psin-ef2 vector
WebApr 27, 2024 · The DAB2IP expression construct was generated by subcloning PCR-amplified full-length human DAB2IP complementary DNA into the pSin-EF2-puro lentiviral vector (Addgene, beijing, China) by EcoRI and NheI. pSin-EF2-puro-DAB2IP or the empty lentiviral vector and 2 packaging plasmids pM2.G and psPAX2 (Addgene, beijing, China) … WebOct 24, 2012 · These transgene-carrying iPSCs (pSin-EF2-gene-Pur vector system) underwent massive cell death (data not shown) after differentiation toward MSC lineages using a reported protocol established for deriving MSCs from hESCs . We used the same protocol to guide hESCs (H1 and H9) and were successful in generating MSC-like cells …
Psin-ef2 vector
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WebOct 5, 2024 · To determine that whether LASP1 is a direct target of miR-203a-3p, CNE-2 and SUNE-1 cells were co-transfected with miR-203a-3p mimic or miR-Ctrl (50 nM, RiboBio) and the pSin-EF2-puro-LASP1 (LASP1) or empty pSin-EF2-puro-Vector (Vector) (2 μg; Addgene, Cambridge, MA, USA). The cells were harvested for assays 48hs after transfection. WebOct 19, 2024 · The pSin-EF2-puro-ZNF671-HA or pSin-EF2-puro-vector plasmids were obtained from Land. Hua Gene Biosciences (Guangzhou, China); pSin-EF2-puro-Vector …
WebMay 11, 2024 · abstract type AbstractPolynomial end """ Polynomial <: AbstractPolynomial Polynomials written in the canonical form --- Polynomial(v::T) where T<:Union{Vector{<:Real}, NTuple{<:Any, <:Real}}) Construct a `Polynomial` from the list of the coefficients. The coefficients are assumed to go from power 0 in the ascending order. WebSystemic delivery of pSin-EF2-ELMO1-Pur facilitates continuous ELMO1 expression in colonic tissues. [A] Immunohistochemical staining of ELMO1 in the colons of untreated mice or mice treated with...
WebJan 7, 2024 · Product Name : pSin-EF2-Sox2-Pur article : bacterial resistance : Ampicillin cloning : growth notes : The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. origin : 37 pi : plasmid copy : SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone. WebpSin4-EF2-IRES-Pur (Search Vector Database) Backbone size w/o insert (bp) 7500 Vector type Mammalian Expression, Lentiviral Selectable markers Puromycin Growth in Bacteria … Comprehensive molecular biology blog covering topics such as plasmids, viral ve…
WebJan 7, 2024 · product information Catalog Number : 16579 Product Name : pSin-EF2-Oct4-Pur article : bacterial resistance : Ampicillin cloning : growth notes : The psPAX2 …
WebJul 18, 2024 · The full length of CTBP1-DT was generated by subcloning the PCR-amplified human CTBP1-DT sequence and cloned into pSin-EF2-vector. The DDUP ORF with FLAG-tag expression plasmid was generated by cloning the full length of CTBP1-DT. heating curve notes pdfWebConstructs expressing eGFP from the pSin- EF2-GFP-Puro based vector were used in subsequent studies. A key bottleneck in many stem cell applications is the ability to identify, select or ... heating curve graph utWebFeb 3, 2024 · The AKR1B10 CDS (coding DNA sequence) was cloned into the lentiviral expression vector pSIN-EF2-Puro (hereinafter referred to as pSIN-EF2) to construct the lentiviral vector pSIN-EF2/AKR1B10. The method to establish stable AKR1B10-expression cell line has been described previously 23. AKR1B10-shRNA stable transfection using … heating curve in chemistryWebFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between … heating curve heat of fusionWebThe empty pSin-EF2 vector (lenti-vector) was used as a control. Observation of morphologic changes. The cells were seeded into six-well culture (Corning Incorporated, Corning, NY, USA) plates and transferred with miR-16 or miR-Ctrl. After incubation for 24 hours, the cellular morphology was observed by phase contrast microscope (Olympus America ... heating curve labeled with equationWebThe psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. These plasmids were created by your colleagues. Please acknowledge the Principal … movie the asphalt jungle 1950WebMar 1, 2024 · Besides, two weeks before DSS administration, the mice were injected with 108 MOI pSin-EF2-REG1A-pur or control pSin-EF2-vector-pur by tail vein every three days. And the mice were sacrificed at day 7 and 14 to examine the … movie the assault 2017