Tfeb s142

Author: xwwg

August undefined, 2024

WebS138 and S142 are localized in the proximity of a NES. (B) S138 and S142 of TFEB are evolutionarily conserved in the indicated species. Alignment of the sequences near TFEB … Web8 Sep 2024 · tfeb在s142的磷酸化还由erk2介导。图4 tfeb 结构域结构中的磷酸化位点及其调节作用. 最近研究还发现stub1通过靶向磷酸化的tfeb(s142和s211)进行泛素介导的蛋白酶 …

Molecular basis of CLN2 disease: A review and classification of …

Web17 Aug 2024 · In particular, TFEB S142A showed impaired phosphorylation of S142 and S138; TFEB S138A showed impaired phosphorylation of S138, but normal S142 … Web30 Nov 2024 · Active mTOR on lysosomal surface phosphorylates TFEB at residues S122, S142 and S211. Once phosphorylation of S211 occurs, TFEB is released from the … shoe repair randwick

TFEB; Beyond Its Role as an Autophagy and Lysosomes …

WebS211 in TFEB is responsible for phosphorylation-dependent interaction with YWHA/14-3-3 proteins. It has been reported that phosphorylation of TFEB at S142 and/or S211 is … Web1 Jan 2015 · Phosphoproteomic studies identified at least 10 different phosphorylation sites on the TFEB protein, while mutation analysis suggested that only phosphorylation at Ser211 and Ser142 is critical for TFEB subcellular localization ( Martina et al., 2012, Roczniak-Ferguson et al., 2012, Settembre et al., 2012 ). Web27 May 2024 · It is previously known that phosphorylation of TFEB at two residues, S142 and S211, influences its nuclear localization and activity [ 15, 16 ]. Under conditions of adequate nutrient supply, dephosphorylation of TFEB promotes its nuclear translocation and activation of CLEAR network genes. rachat de credit belfius

Transcriptional regulation of autophagy-lysosomal function in …

Cells Free Full-Text TFEB Biology and Agonists at a Glance - MDPI

HeLa TFEB-GFP cells were seeded in 96-well plates and incubated for 24 h. After incubation cells were treated as described above, rinsed with PBS once, fixed for 10 min with 4% paraformaldehyde and stained with DAPI. For the acquisition of the images, at least ten image fields were acquired per well of the 96 … See more Cells were cultured in the following media: HeLa (ATCC) in MEM (Cat# ECB2071L, Euroclone); HEK293T (ATCC) in DMEM high glucose (Cat# ECM0728L, Euroclone); ARPE19 (ATCC) in DMEM-F12 (Cat# 11320–033, Thermo … See more Reagents used in this study were obtained from the following sources: Antibodies to Phospho-p70 S6 Kinase (Thr389) (1A5) (Cat# 9206) (1:1000), p70 S6 Kinase (Cat# 9202) (1:1000), 4E-BP1 (Cat# 9644) (1:1000), Phospho-4E … See more The plasmid-encoding ΔNLS-TFEB-GFP was a kind gift of Shawn Ferguson (Yale School of Medicine, CT, USA). TFEB S138A-GFP was a … See more For experiments involving amino acid starvation, cell culture plates were rinsed twice with PBS and incubated in amino acid-free RPMI (Cat# R9010-01, USBiological) supplemented with 10% dialyzed FBS for 60 … See more WebNotably, the level of phosphorylation at S122, S134, S138, and S142 did not decline on TFEB from TSA-treated cells compared with TFEB from DMSO-treated cells (Fig. 5F). We also … shoe repair rapid city sdWebThe O/A-dependent decrease in phospho-S142 corresponds with the shift in MM of TFEB caused by O/A alone and may be the result of the O/A-dependent effects on both AMPK and mTORC1 activity, which likely reflect a loss of mitochondrial function that is independent of mitophagy. Thus, phosphorylation changes at S142 and S211 alone are not sufficient to … shoe repair rancho mirage

"Web2 Feb 2024 · A Representative Western blots showed the levels of phosphorylated (p-) TFEB (S142) and the lysosome marker LAMP1 in the hippocampi of mice from each group. B , C … " - Tfeb s142

Molecular basis of CLN2 disease: A review and classification of …

TFEB; Beyond Its Role as an Autophagy and Lysosomes …

Tfeb s142

Did you know?